Overview
Hands-on workshop on targeted metabolomics, untargeted metabolomics and imaging metabolomics for 21-30 students and postdoctoral scientists. Three sessions of hands-on training, each has 7 – 10 people for 4 hours
Session 1, targeted metabolomics on 4000QTRAP:
- First hour: lecture on MRM-based targeted metabolomics, including sample prep/metabolite extraction, absolute quantification and relative quantification, protocol on metabolite optimization, and quantitative analysis
- Second to third hour: each person optimizes a metabolite
- Last hour: data analysis, and discussion
Session 2, untargeted metabolomics/lipidomics on Orbitrap:
- First hour: lecture on untargeted metabolomics/lipidomics, including sample prep/metabolite extraction, LC-MS2 and MS3 methodology, software and quantitative analysis etc.
- Second to third hour: LC-MS 15 min/each run
- Last hour: data analysis on CD3.1 and lipid search, and discussion
Session 3, imaging metabolomics (spatialOMx) on MALDI-FTICR/timsTOF Flex:
- First hour: lecture on mass spectrometry imaging, including sample prep, imaging methodology, software and quantitative analysis etc.
- Second to third hour: Imaging of plant roots and tissue sections
- Last hour: data analysis, and discussion
Technical symposium is designed to reinforce the hands-on experience and introduce state-of-the-art metabolomics technologies, including single-cell and imaging mass spectrometry, and their applications and potential impact in advancing biological and medical research.
Scientific symposium is designed to focus on discovery and hypothesis-testing research using specific metabolomics technologies in solving important biological questions. Topics related to single-cell metabolomics, multi-omics, discovery of novel natural products, systems biology, and synthetic biology will be covered.
Tentative Schedule
May 6 Hands-on Metabolomics Workshop
ICBR Conference Room (ICBR 189), Proteomics Core (ICBR 169), Medicine (Room M641c) and Chemistry Leigh Hall 214
| Time | Activity | Location | |
| 8:00 – 8:30 | Check in and coffee | 189 | |
| 8:30 – 8:40 | Welcome Introductions
Logistics/Questions |
Dr. Sixue Chen
Dr. Sally Assmann Dr. Tim Garrett Dr. Boone Prentice |
189 |
| 8:40 – 9:00 | Metabolomics Introduction | Dr. Tim Garrett | 189 |
| 9:00 – 9:15 | Three co-current workshop sessions | ||
| 9:15 –12:00 | Session 1: Targeted metabolomics on 4000QTRAP LC-MS/MS
(multiple reaction monitoring) |
Dr. Islam Mostafa
Dr. Wei Zhu Dr. Tim Garrett |
169 and M641c |
| 9:15 –12:00 | Session 2: Untargeted metabolomics on Orbitrap Fusion LC-MS/MS
(including lipidomics) |
Dr. Craig Dufresne; Dr. Kate Erickson Dr. Jin Koh Dr. Tim Garrett | 169 and M641c |
| 9:15 –12:00 | Session 3: Imaging metabolomics (spatialOMx) on MALDI FT-ICR/timsTOF Flex | Dr. Boone Prentice; Bowen Tan | 214 |
| 12:00 – 1:30 | Lunch discussion (pizza) | Dr. Sally Assmann | 189 |
| 1:30 –5:00 | Session 2: Targeted metabolomics on 4000QTRAP LC-MS/MS
(multiple reaction monitoring) |
Dr. Islam Mostafa
Dr. Wei Zhu Dr. Tim Garrett |
169 and M641c |
| 1:30 –5:00 | Session 3: Untargeted metabolomics on Orbitrap Fusion LC-MS/MS
(including lipidomics) |
Dr. Craig Dufresne; Dr. Kate Erickson Dr. Jin Koh Dr. Tim Garrett | 169 and M641c |
| 1:30 –5:00 | Session 1: Imaging metabolomics (spatialOMx) on MALDI FT-ICR/timsTOF Flex | Dr. Boone Prentice; Bowen Tan | 214 |
| 5:00 – 6:30 | Dinner (Asian food) | pickup | 189 |
May 7 morning Hands-on Metabolomics Workshop
| Time | Activity | Location | |
| 8:00 – 8:30 | Breakfast and coffee | 189 | |
| 8:30 – 12:00 | Session 3: Targeted metabolomics on 4000QTRAP LC-MS/MS
(multiple reaction monitoring) |
Dr. Islam Mostafa Dr. Wei Zhu Dr. Tim Garrett | 169 and M641c |
| 8:30 – 12:00 | Session 1: Untargeted metabolomics on Orbitrap Fusion LC-MS/MS
(including lipidomics) |
Dr. Craig Dufresne Dr. Kate Erickson Dr. Jin Koh Dr. Tim Garrett | 169 and M641c |
| 8:30 – 12:00 | Session 2: Imaging metabolomics (spatialOMx) on MALDI FT-ICR/timsTOF Flex | Dr. Boone Prentice; Bowen Tan | 214 |
| 12:00 – 1:00 | Lunch discussion (box lunch) | Dr. Sixue Chen | 189 |
May 7 afternoon Technical Symposium
Genetics Institute Auditorium, Room 101
| Metabolomics Technologies and Applications | ||
| 1:00 – 2:00 | Symposium check in, and putting up poster | |
| 2:00 – 2:45 | Imaging mass spectrometry in biomedical research | Dr. Boone Prentice
University of Florida |
| 2:45 – 3:30 | Orbitrap MS in metabolomics applications | Dr. Craig Dufresne
Thermo Scientific Inc. |
| 3:30 – 3:45 | Break | |
| 3:45 – 4:30 | TBD | |
| 4:30 – 4:50 | TBD | Student speaker 1 |
| 4:50 – 5:10 | TBD | Student speaker 2 |
| 5:10 – 7:00 | Mixer, Vendor Show/Poster view | |
May 8 Scientific Symposium, Genetics Institute, Room 451
| Time | Title | Speaker |
| Morning session (Chair: Dr. Sally Assmann) | ||
| 8:00 | Welcome | Dr. Sally Assmann
Penn State University |
| 8:15 | Multi-omics of plant stress response | TBD |
| 9:00 | Metabolomics revealed marine microbial interactions that mediate carbon, sulfur, and energy cycles | Dr. Bryn Durham
University of Florida |
| 9:45 | Lipidomics of fish response to environmental toxins | Dr. Nancy Denslow
University of Florida |
| 10:30 | Break (Lower Atrium) | |
| 11:00 | Using GC-MS to investigate the production and function of maize volatiles in plant-insect interactions | Dr. Anna Block
USDA ARS |
| 11:45 | Metabolomics of red-light-induced stomatal opening in Arabidopsis thaliana | Dr. Sally Assmann
Penn State University |
| 12:30 | Lunch, Vendor show and Poster view | |
| Afternoon session I (Chair: Dr. Bryn Durham) | ||
| 2:00 | Crosstalk of acidic phytohormones in plant defense | TBD |
| 2:45 | TBD (on imaging mass spectrometry) | Dr. Tim Garrett
University of Florida |
| 3:30 | TBD | TBD |
| 4:15 | Future of Metabolomics, Discussion | Dr. Sixue Chen |
| 5:00 | Departure | |