Metabolomics

All the symposium participants are encouraged to submit abstracts. In addition to invited speakers, three or four 20-minute talks will be selected from submitted abstracts. Presenters of contributed talks will be notified by April 24^th, 2020. Acceptable files for abstract submission are word documents. Please follow strictly the format in the template here.

If you are interested in presenting a poster and/or a short talk, please indicate this at the bottom of your abstract. Please send your abstract in word format by e-mail to Dr. Wei Zhu:

Dr. Wei Zhu
Email: zhu.wei@ufl.edu
Phone: 352-273-8103

Deadline to submit abstracts: April 24, 2020

---

Abstract Submission Template

To be included in program, abstracts should be received no later than April 24th, 2020.

Abstracts are limited to one page with 1 inch margins on all sides. Name of presenting author should be underlined and e-mail of the presenting author should be included. A template is provided below. Title should be in 14 pt arial, authors in 12 pt arial, affiliation and addresses in 10 pt arial. Abstract text in 12 pt arial font. Electronic submission should be to: zhu.wei@ufl.edu

Proteomics of Arabidopsis Redox Proteins in Response to MeJA

Sixue Chen^1*, Sophie Alvarez¹, Mengmeng Zhu¹, Ning Zhu¹, Johanna Strul²

¹Department of Botany and Zoology, University of Florida, Gainesville, FL 32610, USA

²Life for Science Program, University of Florida, Gainesville, FL 32610, USA

* e-mail: schen@ufl.edu

Protein redox regulation is increasingly recognized as an important switch of protein activity in yeast, bacteria, mammals and plants. In this study, we identified proteins with potential thiol switches involved in jasmonate signaling, which is essential for plant defense. Methyl jasmonate (MeJA) treatment led to enhanced production of hydrogen peroxide in Arabidopsis leaves and roots, indicating in vivo oxidative stress. With monobromobimane (mBBr) labeling to capture oxidized sulfhydryl groups and 2D gel separation, a total of 35 protein spots that displayed significant redox and/or total protein expression changes were isolated. Using LC-MS/MS, the proteins in the spots were identified. By comparative analysis of mBBr and SyproRuby gel images, we were able to differentiate proteins that were redox responsive from proteins that displayed abundance changes in response to MeJA. Identification of redox proteins and their cysteine residues involved in the redox regulation allows for a deeper understanding of the jasmonate signaling networks.

Please select one or both:  

1. I will bring a poster

1. I would like to be considered for a short-talk (20 min)

 

Poster Presentation